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Molecular cytogenetic identification of a wheat-Thinopyrum ponticum substitution line with stripe rust resistance

机译：具有抗条锈性的小麦-Thonpyrum ponticum代换系的分子细胞遗传学鉴定

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Thinopyrum ponticum (2n = 10x = 70) has donated rust resistance genes to protect wheat from this fungal disease. In the present study, the line ES-7, derived from the progeny of the crosses between common wheat cultivar Abbondanza and Triticum aestivum–Th. ponticum partial amphiploid line Xiaoyan784, was characterized by cytological, fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and EST-STS marker techniques. Cytological observations revealed that the configuration of ES-7 was 2n = 42 = 21 II. GISH and FISH results showed that ES-7 had two St chromosomes and lacked 5A chromosomes compared to common wheat. The 4A chromosome of ES-7 had small alterations from common wheat. Two EST-SSR markers BE482522 and BG262826, specific to Th. ponticum and tetraploid Pseudoroegneria spicata (2n = 4x = 28), locate on the homoeologous group 5 chromosomes of wheat, could amplify polymorphic bands in ES-7. It was suggested that the introduced St chromosomes belonged to homoeologous group 5, that is, ES-7 was a 5St (5A) disomic substitution line. Furthermore, ES-7 showed highly resistance to mixed stripe rust races of CYR32 and CYR33 in adult stages, which was possibly inherited from Th. ponticum. Thus, ES-7 can be used for wheat stripe rust resistance breeding program.
机译：ponticum（2n = 10x = 70）捐赠了防锈基因，以保护小麦免受这种真菌病的侵害。在本研究中，品系ES-7来源于普通小麦品种Abbondanza和小麦（Triticum aestivum–Th）杂交的后代。桥本部分二倍体系Xiaoyan784的特征在于细胞学，荧光原位杂交（FISH），基因组原位杂交（GISH）和EST-STS标记技术。细胞学观察显示ES-7的构型为2n ＝ 42 ＝ 21II。 GISH和FISH结果表明，与普通小麦相比，ES-7具有两条St染色体，缺乏5A染色体。 ES-7的4A染色体与普通小麦相比有很小的变化。 Th特异的两个EST-SSR标记BE482522和BG262826。在小麦的第5组同源染色体上发现的斑马和四倍体假单胞菌（2n = 4x = 28），可以扩增ES-7的多态性条带。提示引入的St染色体属于同源5组，即ES-7是5St（5A）二体组置换系。此外，ES-7对成年期的CYR32和CYR33混合条纹锈病显示出高度抗性，这可能是从Th遗传而来的。 ponticum。因此，ES-7可用于小麦条纹抗锈育种计划。

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